Coding

Part:BBa_K3285700:Design

Designed by: Abhinav Masih   Group: iGEM19_IISER-Pune-India   (2019-10-21)


Lead Sequestration Protein with RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 532
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was synthesised with the RBS and prefix and suffix included


Source

pbrD is present in the pbr (lead resistance) operon from the pMOL30 chromosome of Cupriavidus metallidurans CH34 strain.

References